밤파일 만들때 레퍼런스랑 지금 돌리는 레퍼런스랑 다를 경우 발생되는 에러이다.


<에러메세지>
org.broadinstitute.sting.utils.exceptions.ReviewedStingException: Invalid sequence number 24
 at org.broadinstitute.sting.gatk.datasources.reads.GATKBAMIndex.<init>(GATKBAMIndex.java:100)
 at org.broadinstitute.sting.gatk.datasources.reads.BAMSchedule.<init>(BAMSchedule.java:105)
 at org.broadinstitute.sting.gatk.datasources.reads.BAMScheduler.getNextOverlappingBAMScheduleEntry(BAMScheduler.java:205)
 at org.broadinstitute.sting.gatk.datasources.reads.BAMScheduler.advance(BAMScheduler.java:108)
 at org.broadinstitute.sting.gatk.datasources.reads.BAMScheduler.next(BAMScheduler.java:79)
 at org.broadinstitute.sting.gatk.datasources.reads.BAMScheduler.next(BAMScheduler.java:47)
 at net.sf.picard.util.PeekableIterator.advance(PeekableIterator.java:71)
 at net.sf.picard.util.PeekableIterator.next(PeekableIterator.java:57)
 at org.broadinstitute.sting.gatk.datasources.reads.LowMemoryIntervalSharder.next(LowMemoryIntervalSharder.java:61)
 at org.broadinstitute.sting.gatk.datasources.reads.LowMemoryIntervalSharder.next(LowMemoryIntervalSharder.java:36)
 at org.broadinstitute.sting.gatk.datasources.reads.LocusShardStrategy.next(LocusShardStrategy.java:142)
 at org.broadinstitute.sting.gatk.datasources.reads.LocusShardStrategy.next(LocusShardStrategy.java:42)
 at org.broadinstitute.sting.utils.threading.GenomeLocProcessingTracker$OwnershipIterator$1.doBody(GenomeLocProcessingTracker.java:258)
 at org.broadinstitute.sting.utils.threading.GenomeLocProcessingTracker$OwnershipIterator$1.doBody(GenomeLocProcessingTracker.java:250)
 at org.broadinstitute.sting.utils.threading.GenomeLocProcessingTracker$WithLock.run(GenomeLocProcessingTracker.java:423)
 at org.broadinstitute.sting.utils.threading.GenomeLocProcessingTracker$OwnershipIterator.next(GenomeLocProcessingTracker.java:250)
 at org.broadinstitute.sting.utils.threading.GenomeLocProcessingTracker$OwnershipIterator.next(GenomeLocProcessingTracker.java:213)
 at org.broadinstitute.sting.gatk.executive.LinearMicroScheduler.execute(LinearMicroScheduler.java:53)
 at org.broadinstitute.sting.gatk.GenomeAnalysisEngine.execute(GenomeAnalysisEngine.java:235)
 at org.broadinstitute.sting.gatk.CommandLineExecutable.execute(CommandLineExecutable.java:117)
 at org.broadinstitute.sting.commandline.CommandLineProgram.start(CommandLineProgram.java:221)
 at org.broadinstitute.sting.gatk.CommandLineGATK.main(CommandLineGATK.java:87)

==>

This is a terrible error message with what I suspect is a fairly simple cause: I think you're running with a different reference (or different version of the same reference) than the reference to which your BAMs were initially aligned. Can you check to make sure that the version of the reference you're using matches the BAMs exactly?
(http://getsatisfaction.com/gsa/topics/unifiedgenotyper_problem_with_nt_option)
Posted by 옥탑방람보
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